Vitamin E may be useful in maintaining bone quality

A study led by researchers from Toyo University in Japan have found that a vitamin E derivative called tocotrienol-rich fraction (TRF) can effectively inhibit the activation of Janus kinase (JAK) signaling — which regulates the lysyl oxidase (LOX) enzyme in osteoblastic cells. The study, published in the Journal of Nutrition & Intermediary Metabolism, looked at the link between TRF and LOX expression and how it affects bone quality.

  • Studies have shown that JAK signaling is located in the upstream of LOX and that its activation prevents LOX expression.
  • Researchers sought to understand the link between the effect of TRF in LOX expression in cells that are in charge of bone development (osteoblastic cells).
  • In the study, the team cultured an MG-63 human osteosarcoma cell line. Mediums that used 5 micrograms per milliliter (mcg/mL) and 10 mcg/mL of TRF were used for the study, both of which were applied to the cell lines.
  • Twenty-four hours after treatment, the LOX mRNA expression and JAK1, JAK2 protein expression and activation were analyzed. To determine mRNA expression, as well as protein expression and activation, the team used real-time PCR and western-blotting assays, respectively. The team then compared expression and extraction levels between TRF-treated and untreated control groups. In addition, Dunnet’s test was performed to determine statistical analysis.
  • Cells from the TRF-treated group exhibited an increase in LOX mRNA expression and a decrease in JAK2 protein expression and activation. JAK1 expression and activation did not change.

From the findings, the researchers concluded that TRF has the potential to prevent bone deterioration.

Learn more about how vitamin E can improve bone health at

Journal Reference:

Kono K, Yamada W, Ota M, Yano T. Kono K, Yamada W, Ota M, Yano T. EFFECTS OF TOCOTRIENOL-RICH FRACTION ON PREVENTION OF DETERIORATION OF BONE QUALITY. Journal of Nutrition & Intermediary Metabolism. 2016;4:43. Journal of Nutrition & Intermediary Metabolism. 23 April 2016;4:43. DOI: 10.1016/j.jnim.2015.12.312

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